First Case Report of Rabies in a Wolf (Canis Lupus Pallipes) from India

نویسنده

  • Nithin Prabhu
چکیده

This is the first documented evidence of rabies in the Indian wolf (Canis lupus pallipes) to date. The study describes the laboratory confirmation of rabies in wolf from the southern Indian state of Karnataka by the direct fluorescent antibody (DFA) test and a direct rapid immunohistochemistry test (dRIT). The PCR amplified products of complete N and G genes of this wolf RABV were sequenced and processed. The BLAST results and phylogenetic analysis revealed high homology and genetic relatedness of this RABV isolate with those from nearby geographic locations such as in India (human, buffalo), Pakistan (wolf, cattle) and Nepal (goat). Analysis of the deduced amino acid sequences of the complete N and G proteins revealed that wolf RABV isolate recovered in this study belonged to the Arctic-like lineage, which is wide spread throughout the Indian subcontinent, and the region. These findings emphacise the necessity of epidemiological surveillance to characterize regional lyssaviruses to ascertain if wolves (and other wild species) serve as a significant population for RABV enzootic maintenance in India.

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تاریخ انتشار 2014